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Mycobacterium tuberculosis Susceptibility Testing

Use of Test

Antibiotic susceptibility testing of Mycobacterium tuberculosis

Test Includes

BD BACTEC™ MGIT™ Antimycobacterial Susceptibility Testing (AST) for the following antituberculous drugs: Streptomycin, Isoniazid, Rifampin, Ethambutol, and Pyrazinamide


Test is available to all clinical laboratories and local public health jurisdictions.


Susceptibility testing is only performed on Mycobacterium tuberculosis.


Public health investigation testing - Exempt
Non-public health investigation testing - Exempt

Causes for Rejection

Inappropriate specimen transport container; Unlabeled or mislabeled specimen; Inappropriate transport conditions; Incomplete or missing specimen submission form; Specimen leakage during transport; Inappropriate specimen for test ordered; Specimen outdated, exceeds appropriate time from collection to receipt; Quantity not sufficient to perform test; Improperly preserved specimen

Turnaround Time

14 days from identification (isolates),
17 days from identification (clinical)

Forms Required

Microbiology Submission Form (HEA 2530)

Specimen Requirements

Pure subculture of Mycobacterium tuberculosis on agar slant ≤ 14 days. MGIT positive within 5 days of culture positive or liquid medium to support organism growth.

Shipping Requirements

Ship overnight at ambient temperature in compliance with federal regulation and local guidelines. Refer to the Shipping Resources page for more information.